Code: CTQ-024


Title: Instrumentation Applied to Molecular Spectroanalytical Techniques


Level: Master / PhD

Mandatory: no

Total study time: 96h                                                   TPI: 2-2-4


Introduction to molecular absorption and emission spectrometry. Concepts and limitations of Beer´s Law. Applications and advances of molecular spectroanalytical techniques. Introduction to instrumentation (light sources, wavelength selectors and detectors) and to interferences in chemical analysis using UV/Vis spectrophotometry and luminescence. Automation of analytical procedures. Flow injection analysis (fundamentals and applications) and basic analytical instrumentation for automation of chemical analysis (basic electronics and interfacing).


  • D. A. Skoog, F. J. Holler, R. C. Stanley, Principles of Instrumental Analysis, Brooks Cole. 2006.
  • D. C. Harris, Quantitative Chemical Analysis, W.H.Freeman & Co Ltd, 7 ed, 2006.
  • L. Wilson, Quantitative Analysis: Gravimetric, Volumetric and Instrumental Analysis, Mohican Publishing, 2000.
  • K. Van Dyke, C. Van Dyke, K. Woodfork, Luminescence Biotechnology Instruments and Applications, CRC Press, 2001.

Additional bibliography

  • A. M. Garcia-Campana, Chemiluminescence in Analytical Chemistry, CRC, 2001.
  • E. Pungor, G. Horvai, A Practical Guide to Instrumental Analysis, CRC, 1994.
  • L. Kricka (Editor), Analytical Applications of Bioluminescence and Chemiluminescence, Academic Press, 1984.
  • P. S. Stanley (Editor), L. Kricka (Editor), Bioluminescence & Chemiluminescence: Progress & Current Applications, World Scientific Publishing Company, 2002.
  • C. Ronda, ed. Luminescence: from theory to applications, Wiley, 2008.